In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RBRC09435 Iwama, Atsushi C (3-6 months) Bacteriophage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Stop (synthetic), FLAG tag (synthetic), mouse ESET cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Aequorea victoria EGFP cDNA, mouse Gt(ROSA)26Sor genomic DNA B6-Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)> B6-Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)> Rosa26-3xFlag-ESET-IRES-GFP- KI Rosa26-3xFlag-ESET-IRES-GFP- KI 条件を付加する。<br>研究成果の公表にあたって謝辞の表明を必要とする。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> 岩間 厚志 129/B6 F1 ES細胞から作製され以後C57BL/6にbackcrossを6回行った。造血系に発現させた場合には明らかな表現系は認められないが、他に関しては調べていない。 Fluorescent Proteins/lacZ System Eset/Setdb1を組織・細胞特異的に発現させる。 C(3〜6か月) <a href='https://brc.riken.jp/mus/pcr09435'>Genotyping protocol -PCR-</a> true Cre/loxP system